![]() ![]() A significant increase in the number of dead or dying SCs was observed in nerve segments cultured for 24 hours. p-ERK1/2 and m-calpain were also observed in axons. The experiments revealed that immunoreactivity for p-ERK1/2 increased with time in organotypically cultured SCs. Immunohistochemistry of sections of the cultured nerve segments was performed to label m-calpain and the phosphorylated and activated form of ERK1/2. In some experiments, 5-bromo-2’-deoxyuridine (BrdU) was added during the last 24 hours to detect proliferating cells and propidium iodide (PI) was added at the last hour to detect dead and/or dying cells. Nerve segments were cultured for up to 72 hours with and without ethylene glycol-bis(β-aminoethyl ether)-N, N, N’, N’-tetraacetic acid (EGTA). Here, we investigated axotomy-induced activation of extracellular-signal-regulated kinase-1/2 (ERK1/2 important for proliferation) and m-calpain in vitro, and the relation to Ca 2+ deletion and Schwann cell proliferation and death after rat sciatic nerve axotomy. Detailed mechanisms behind regeneration after nerve injury, in particular signal transduction and the fate of Schwann cells (SCs), are poorly understood. ![]()
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